The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

The 2017/18  impact factor of the Herpetological Journal is 1.268

pdf 05. Artificial reproduction using leuprolide acetate in the frog Rana pipiens


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pp. 125-130

Authors: Laura A. Brannelly, Michel E. B. Ohmer & Corinne L. Richards-Zawacki

Abstract: Procuring offspring from captive animals can be important for research and conservation efforts. Yet, reliable methods for obtaining fertilised eggs are unavailable for many species. In this study, we examined the efficacy of one drug, leuprolide acetate, to induce reproduction (i.e. egg production, tadpole hatching) in the northern leopard frog, Rana pipiens. We found that leuprolide acetate successfully induced breeding and larval development in animals that were overwintered in the lab, but not in animals caught during the breeding season. These results indicate that leuprolide acetate can be successful in inducing breeding and fertilisation of frog eggs, but that its effectiveness might be contingent upon length of time in captivity and the animals having undergone an artificial overwintering period in the laboratory prior to induction. Artificial breeding success is species and context dependent; therefore, identifying additional methods effective across taxa will help support species in need of management intervention.

Keywords: Amphibian; Amphibian reproduction; Assisted reproductive technologies; Breeding; Hormone induction; Leuprolide Acetate; Spawning

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