The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

The 2017/18  impact factor of the Herpetological Journal is 1.268

pdf 05. A new method for quantitative pattern analysis applied to two European Bombina species


Open Access

pp. 97-103
Authors: Vörös, Judit; Szalay, Ferenc & Barabás, Lilla

Abstract: This paper describes a new computer-aided digital image analysing method with self-developed modifications of public domain software (Scione Image 4.02b) for quantification of geometric and colour-defined characters of patches. We demonstrate the method on Bombina bombina and B. variegata and their natural hybrids, separating the two species and defining intermediate specimens according to their belly colour pattern. Belly images of 20 B. bombina, 20 B. variegata and five of their hybrids were processed and analysed, using four patch size- and shape-related characters (patch density, area ratio, ratio of mean patch area and mean patch perimeter, circularity) and three colour-related characters (red, green and blue components). Descriptive statistics (Student's t-test) and canonical variate analysis were used to discriminate among the three groups of specimens, and the results were compared with results based on independent genetic data. The two species significantly differed in all the geometric and two of the colour characters, and were clearly separated by the multivariate analysis, while the hybrid specimens had intermediate features.


Download Access:

The latest 20 issues can be downloaded when logged in with a Herpetological Journal subscription membership.

Individual articles can be purchased for download.

Older issues and occasional Open Access articles are available for public download


For further information and submission guidelines please see our Journal Instructions to Authors

pdfBHS Ethics Policy

NOTE: as of January 2017, all new editions of the HJ are ONLY available online via the BHS website. The BHS no longer has a commercial hosting agreement with Ingenta  -  although editions prior to end 2016 remain accessible on Ingenta .  Those editions are of course also accessible on the BHS website for subscribers with an active and valid membership.  Should you experience any difficulty accessing HJ editions via the website or have any queries in this regard, please contact