The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

 ISSN 0268-0130

2021 Impact Factor from Clarivate for the Herpetological Journal is 1.194, an increase of 0.332 from 2020.

pdf 05. Effects of low temperature on testicular cells in the marbled newt, Triturus marmoratus (Caudata, Salamandridae)


Open Access


Authors: Francisco J. Saez, Benito Fraile, Mary Paz De Miguel And Ricardo Paniagua

Abstract: The response of the different germ cell types and glandular tissue of the testis to low temperatures (4°C) and long photoperiods ( 16L:8D) was studied in the marbled newt (Triturus marmoratus) by histologic quantitative methods in the three periods of the annual cycle: quiescence (January-March), germ cell proliferation up to round spermatids (April-June), and spermiogenesis (July-September). Together with each group of cold-exposed newts, another group was maintained at mild temperature (20°C) over the same long photoperiod. At the beginning and end of each period, initial and final wild controls were collected. In the quiescent period, only spermatogonial proliferation was observed in the initial and final controls as well as in the cold-exposed newts. The newts kept at 20°C developed spermatogenesis up to the round spermatid level. At the end of the germ cell proliferation period, the final controls showed round spermatids; the newts exposed to 20°C developed complete spermatogenesis; and the newts maintained at 4°C only presented spermatogonial proliferati_on. At the end of the spermiogenesis period, the final controls and the newts kept at 20°C showed complete spermatogenesis and developed glandular tissue whereas the newts exposed to 4°C only had round spermatids and had no glandular tissue. Present results suggest that although low temperature does not affect spermatogonium proliferation it impedes both the subsequent steps in spermatogenesis and the development of glandular tissue.

Download Access:

The latest 8 issues can be downloaded when logged in with a Herpetological Journal subscription membership.

Individual articles can be purchased for download.

Older issues and occasional Open Access articles are available for public download


For further information and submission guidelines please see our Journal Instructions to Authors

pdfBHS Ethics Policy


Please note that as from Volume 31 Number 1 (January 2021) on, the Herpetological Journal will be available as an online publication only - the last print edition will be Volume 30 Number 4.   

Aligning with this change, it is now no longer possible to purchase a subscription that includes a print copy of the HJ.  All members who have existing HJ print subscriptions that remain active as at end June 2020 will receive the full four 2020 print editions.  New subscribers or renewals after this time will only have option to subscribe to the online only subscription package.  Subscription pricing has been amended to reflect the content changes.