The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

The 2017/18  impact factor of the Herpetological Journal is 1.268


pdf 09. Characterization of nine polymorphic microsatellite loci in the dyeing poison frog Dendrobates tinctorius (Dendrobatidae), and their cross-species utility in two other dendrobatoid species

302 downloads

Open Access

pp. 263-265
Authors: Ringler, Eva; Rojas, Bibiana; Ringler, Max & Hödl, Walter

Abstract: While field and laboratory based studies have provided significant insights into the parental care and courtship behaviour of dendrobatoid frogs, a comprehensive assessment of their genetic mating systems and population genetic parameters has been precluded because of the lack of highly variable DNA markers. Here we document the development of nine novel polymorphic microsatellite markers for the dyeing poison frog Dendrobates tinctorius (Dendrobatidae). We found between three and 16 alleles per locus in 60 individuals (30 males, 30 females) from the field site Saut Pararé, French Guiana, with an average observed heterozygosity of 0.75. None of the loci deviated significantly from Hardy–Weinberg equilibrium or showed linkage disequilibrium. We also report successful cross-species amplification of the nine markers in two other dendrobatoid species (Allobates femoralis and Oophaga pumilio). These markers have the potential to aid in determining the genetic structure of local populations, identifying small-scale phylogenies such as parent-offspring relationships and will allow for cross-species comparisons within dendrobatoid species. Therefore, these markers can be applied to a wide range of scientific fields, such as conservation, behavioural ecology and evolutionary biology.

Keywords: MICROSATELLITE MARKER, DENDROBATES TINCTORIUS, DENDROBATIDAE, CROSS-SPECIES AMPLIFICATION, POLYMORPHIC

Download Access:

The latest 20 issues can be downloaded when logged in with a Herpetological Journal subscription membership.

Individual articles can be purchased for download.

Older issues and occasional Open Access articles are available for public download

Submissions:

For further information and submission guidelines please see our Journal Instructions to Authors

pdfBHS Ethics Policy

NOTE: as of January 2017, all new editions of the HJ are ONLY available online via the BHS website. The BHS no longer has a commercial hosting agreement with Ingenta  -  although editions prior to end 2016 remain accessible on Ingenta .  Those editions are of course also accessible on the BHS website for subscribers with an active and valid membership.  Should you experience any difficulty accessing HJ editions via the website or have any queries in this regard, please contact