The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

The 2017/18  impact factor of The Herpetological Journal is 1.268

ISSN 0268-0130


pdf 08. Whole extra-charged DNA spermatozoa in the saltwater crocodile (Crocodylus porosus) ejaculate

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Open Access

pp. 313-316

Authors: J. Gosálvez, C. López-Fernández & S.D. Johnston

Abstract: Saltwater crocodile spermatozoa examined in this study exhibited unusually high levels of extra DNA charged (2C and 4C) spermatozoa that can be explained by arrested cytokinesis at both meiotic divisions. From the ejaculates of five crocodiles, abnormal spermatozoa containing 2C (1–12%) and 4C DNA (0–2%) charges were discriminated after assessing 1500 spermatozoa using morphological characterisation under fluorescence microscopic and image analysis protocols. Individual crocodiles with a high proportion of 2C spermatozoa in their ejaculate, also showed a high level of DNA fragmentation in 1C sperm cells (8.7– 12.7%) but there was no such relationship between DNA fragmentation and 2C sperm. The value of whole extracharged sperm DNA in the ejaculate as a possible marker for fertility is discussed.

Key words: polyploid sperm, reptile, reproduction, saltwater crocodile, sperm DNA fragmentation

 

 

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IMPORTANT NOTE - JUNE 2020

Please note that as from Volume 31 Number 1 (January 2021) on, the Herpetological Journal will be available as an online publication only - the last print edition will be Volume 30 Number 4.   

Aligning with this change, it is now no longer possible to purchase a subscription that includes a print copy of the HJ.  All members who have existing HJ print subscriptions that remain active as at end June 2020 will receive the full four 2020 print editions.  New subscribers or renewals after this time will only have option to subscribe to the online only subscription package.  Subscription pricing has been amended to reflect the content changes.