The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

 ISSN 0268-0130

2021 Impact Factor from Clarivate for the Herpetological Journal is 1.194, an increase of 0.332 from 2020.

pdf 07.A review of torrent frogs (Amolops: Ranidae) from Bhutan, the description of a new species, and reassessment of the taxonomic validity of some A. viridimaculatus group species aided by archival DNA sequences of century-old type specimens


Open Access


pp: 142-175

Authors: Stephen Mahony, Tshering Nidup, Jeffrey W. Streicher, Emma C. Teeling & Rachunliu G. Kamei

Abstract: Seven species of the Asian torrent frogs (genus Amolops) have previously been reported from the eastern Himalayan country of Bhutan. Species identifications from the region have been largely based on photographed animals with few voucher specimens available and no molecular sampling. Understanding the taxonomic status of Bhutan’s torrent frogs has also been hampered by the poorly understood distributional limits of species from surrounding regions. Herein we utilised molecular phylogenetic and morphological data for vouchered specimens from Bhutan and provide a complete literature review of all Amolops populations reported from the country. Phylogenetic relationships were estimated by combining available sequence data (from GenBank) with newly generated sequences from recently collected Bhutanese Amolops populations. We also obtained archival DNA sequences from the type specimens of Amolops formosus, A. himalayanus, and A. kaulbacki, collected between 82 and 151 years ago. Our comparative analyses revealed a large, new (to science) species of the Amolops viridimaculatus group from eastern Bhutan. Morphological examinations of related taxa revealed that A. senchalensis from India is not a synonym of A. marmoratus. Molecular phylogenetic results supplemented by morphological data unambiguously demonstrate i) that A. himalayanus is present in eastern Nepal, ii) the presence of a previously undocumented population of A. nepalicus in eastern Nepal, iii) a 200 km range extention for A. kaulbacki into Yunnan, China, iv) that A. gyirongensis should be considered a junior subjective synonym of A. formosus, and v) that A. splendissimus from Vietnam should be considered a junior subjective synonym of A. viridimaculatus. Based on our results, we expand the Amolops viridimaculatus group to include nine species, including A. formosus, A. himalayanus, A. kaulbacki, and the new species described herein. We provisionally include a further three species in the viridimaculatus group based on morphology, A. longimanus, A. nidorbellus, and A. senchalensis. Combining our data with the literature review allowed us to identify several unidentified Amolops species from recent phylogenetic studies and remove nine frog species (including Hyla, Sylvirana, and seven Amolops species) from Bhutan’s amphibian checklist. We recognise four species of Amolops in Bhutan, three of which cannot be confidently identified to the species level based on currently available data.

Keywords: Anura, taxonomy, Himalayas, conservation, vouchered-specimens

Download Access:

The latest 8 issues can be downloaded when logged in with a Herpetological Journal subscription membership.

Individual articles can be purchased for download.

Older issues and occasional Open Access articles are available for public download


For further information and submission guidelines please see our Journal Instructions to Authors

pdfBHS Ethics Policy


Please note that as from Volume 31 Number 1 (January 2021) on, the Herpetological Journal will be available as an online publication only - the last print edition will be Volume 30 Number 4.   

Aligning with this change, it is now no longer possible to purchase a subscription that includes a print copy of the HJ.  All members who have existing HJ print subscriptions that remain active as at end June 2020 will receive the full four 2020 print editions.  New subscribers or renewals after this time will only have option to subscribe to the online only subscription package.  Subscription pricing has been amended to reflect the content changes.