The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.
2020 Impact Factor for the Herpetological Journal is 0.862
Authors: Louise Bardsley, Sean Smith And Trevor J . C. Beebee
Abstract: We have characterized two molecular techniques, protein typing and RAPD analysis, for the identification of two species of European Bufo larvae (Bufo bufo and B. calamita). These tadpoles are very difficult to distinguish on morphological grounds. Protein typing was improved by the use of a sensitive (silver-based) staining method, and replacement of low temperatures with ethanol submergence for tissue storage. Both techniques reliably resolved the two species and required very small amounts (<2 mg) of tissue, which could be obtained easily and without sacrificing the animals.