The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

 ISSN 0268-0130

2020 Impact Factor for the Herpetological Journal is 0.862

pdf 03. Identification of Bufo larvae by molecular methods


Open Access


Authors: Louise Bardsley, Sean Smith And Trevor J . C. Beebee

Abstract: We have characterized two molecular techniques, protein typing and RAPD analysis, for the identification of two species of European Bufo larvae (Bufo bufo and B. calamita). These tadpoles are very difficult to distinguish on morphological grounds. Protein typing was improved by the use of a sensitive (silver-based) staining method, and replacement of low temperatures with ethanol submergence for tissue storage. Both techniques reliably resolved the two species and required very small amounts (<2 mg) of tissue, which could be obtained easily and without sacrificing the animals.

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Please note that as from Volume 31 Number 1 (January 2021) on, the Herpetological Journal will be available as an online publication only - the last print edition will be Volume 30 Number 4.   

Aligning with this change, it is now no longer possible to purchase a subscription that includes a print copy of the HJ.  All members who have existing HJ print subscriptions that remain active as at end June 2020 will receive the full four 2020 print editions.  New subscribers or renewals after this time will only have option to subscribe to the online only subscription package.  Subscription pricing has been amended to reflect the content changes.