The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

 ISSN 0268-0130

2021 Impact Factor from Clarivate for the Herpetological Journal is 1.194, an increase of 0.332 from 2020.

pdf 05. Acute toxicity tests on Japanese amphibian larvae using thiobencarb, a component of rice paddy herbicides


Open Access


Authors: Masahiro Saka

Abstract: Acute toxicity tests were carried out on five species of Japanese amphibian larvae, at different developmental stages, to assess the risk posed by thiobencarb, a component of rice paddy herbicides. Test substances were four types of commercially formulated herbicide containing mainly thiobencarb, and the 24 h, 48 h, 72 h and 96 h LC50 (median lethal concentration) values of these herbicides were calculated by probit analysis. These values ranged from 0.9 to 6.5 mg/I of thiobencarb. Newly hatched larvae seemed to be slightly more resistant to the herbicides than well-developed larvae in all test species. There were no clear interspecific differences in responses. The actual thiobencarb concentration in paddy water was measured with indoor models for two weeks, and it ranged from Xenopus laevis produced approximately the same LC50 values as those of Japanese amphibians. This indicates that experimental frogs such as Xenopus laevis can act as a model for these native and wild amphibians when toxicity tests are conducted.

Keywords: Japanese amphibians, herbicide, thiobencarb, acute toxicity, risk assessment

Download Access:

The latest 8 issues can be downloaded when logged in with a Herpetological Journal subscription membership.

Individual articles can be purchased for download.

Older issues and occasional Open Access articles are available for public download


For further information and submission guidelines please see our Journal Instructions to Authors

pdfBHS Ethics Policy


Please note that as from Volume 31 Number 1 (January 2021) on, the Herpetological Journal will be available as an online publication only - the last print edition will be Volume 30 Number 4.   

Aligning with this change, it is now no longer possible to purchase a subscription that includes a print copy of the HJ.  All members who have existing HJ print subscriptions that remain active as at end June 2020 will receive the full four 2020 print editions.  New subscribers or renewals after this time will only have option to subscribe to the online only subscription package.  Subscription pricing has been amended to reflect the content changes.