The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

The 2017/18  impact factor of The Herpetological Journal is 1.268

ISSN 0268-0130


pdf 05. Species assignment in the Pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers

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pp. 99-108

Authors: Gregorio Sánchez-Montes, Ernesto Recuero, Jorge Gutiérrez-Rodríguez, Ivan Gomez-Mestre & Iñigo Martínez-Solano

Abstract: Pelophylax perezi is an Iberian green waterfrog with high tolerance to habitat alteration that at times shows local population growth and demographic expansion, even where other species decline. However, pond destruction, invasive predators and hybridisation with other European waterfrog species (P. ridibundus) threaten many of its populations across its range. Hybrids of P. perezi and P. ridibundus (P. kl. grafi) can breed successfully with the former parental species after discarding the whole P. perezi genome in the germinal line, thus representing a sexual parasite for P. perezi. However, little is known about the extent of the contact zone of this hybridogenetic complex. Due to the morphological similarity of the three taxa, molecular tools are needed to delineate their respective ranges. Here we characterise a set of 16 microsatellite markers specifically developed for P. perezi. These markers showed moderate to high polymorphism (2–17 alleles/locus) in two populations from central Spain (n=20 and n=23), allowing individual identification of frogs. Seven of these markers cross-amplified in individuals of P. ridibundus from southern France (3–8 alleles/locus). These markers were used to genotype samples along a transect from southern France to eastern Spain, encompassing both pure and hybrid individuals. Sample assignment to each taxon was based on the new microsatellite loci and compared with nuclear and mitochondrial sequence data. Our results show that these markers are useful to distinguish P. ridibundus, P. perezi and the hybrid form P. kl. grafi from each other, even when sample sizes are low. The newly characterised markers will also be useful in demographic and phylogeographic studies in P. perezi and are thus a valuable tool for evolutionary and conservation oriented research.

Key words: cross-amplification, hybridisation, microsatellites, Pelophylax kl. grafi, Pelophylax perezi, Pelophylax ridibundus

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IMPORTANT NOTE - JUNE 2020

Please note that as from Volume 31 Number 1 (January 2021) on, the Herpetological Journal will be available as an online publication only - the last print edition will be Volume 30 Number 4.   

Aligning with this change, it is now no longer possible to purchase a subscription that includes a print copy of the HJ.  All members who have existing HJ print subscriptions that remain active as at end June 2020 will receive the full four 2020 print editions.  New subscribers or renewals after this time will only have option to subscribe to the online only subscription package.  Subscription pricing has been amended to reflect the content changes.